Landauer principle on dissipation in the irreversible computations. In our experiment, the qubit is initialized to states according to Gibbs distribution. Work injection and extraction processes are conducted through two kinds of unitary driving protocols, for both a forward process and its corresponding mirror reverses. Relative entropy and relative Re’nyi entropy are employed to measure the asymmetry between paired forward and backward work injection or extraction processes. We show experimentally that relative entropy and relative Re’nyi entropy measured irreversibility are related to the average of work dissipation and average of exponentiated work dissipation respectively. Our work provides solid experimental support for the theory of quantum thermodynamics.