erroneous results during simultaneous operations. For superconducting qubits which operate in the microwave regime, electromagnetic isolation is often limited due to design constraints, leading to signal crosstalk that can deteriorate the quality of simultaneous gate operations. Here, we propose and demonstrate a method based on AC Stark effect for calibrating the microwave signal crosstalk. The method is suitable for processors based on fixed-frequency qubits which are known for high coherence and simple control. The optimal compensation parameters can be reliably identified from a well-defined interference pattern. We implement the method on an array of 7 superconducting qubits, and show its effectiveness in removing the majority of crosstalk errors.