(QEC) to actively protect against both. In the smallest QEC codes, the information in one logical qubit is encoded in a two-dimensional subspace of a larger Hilbert space of multiple physical qubits. For each code, a set of non-demolition multi-qubit measurements, termed stabilizers, can discretize and signal physical qubit errors without collapsing the encoded information. Experimental demonstrations of QEC to date, using nuclear magnetic resonance, trapped ions, photons, superconducting qubits, and NV centers in diamond, have circumvented stabilizers at the cost of decoding at the end of a QEC cycle. This decoding leaves the quantum information vulnerable to physical qubit errors until re-encoding, violating a basic requirement for fault tolerance. Using a five-qubit superconducting processor, we realize the two parity measurements comprising the stabilizers of the three-qubit repetition code protecting one logical qubit from physical bit-flip errors. We construct these stabilizers as parallelized indirect measurements using ancillary qubits, and evidence their non-demolition character by generating three-qubit entanglement from superposition states. We demonstrate stabilizer-based quantum error detection (QED) by subjecting a logical qubit to coherent and incoherent bit-flip errors on its constituent physical qubits. While increased physical qubit coherence times and shorter QED blocks are required to actively safeguard quantum information, this demonstration is a critical step toward larger codes based on multiple parity measurements.