by a classical computer and passed via control hardware to the quantum processor. Here, we demonstrate the first experimental realization of a quantum instruction set, in which a fixed sequence of classically-defined gates perform an operation that is fully determined only by a quantum input to the fixed sequence. Specifically, we implement the density matrix exponentiation algorithm, which consumes N copies of the instruction state ρ to approximate the operation e−iρθ (θ an arbitrary angle). Our implementation relies on a 99.7\% fidelity controlled-phase gate between two superconducting transmon qubits. We achieve an average algorithmic fidelity ≈0.9, independent of the setting of ρ, to circuit depth nearly 90. This new paradigm for quantum instructions has applications to resource-efficient protocols for validating entanglement spectra, principal component analysis of large quantum states, and universal quantum emulation.