Two-level systems (TLS) of unclear physical origin are a major contributor to decoherence in superconducting qubits. The interactions of individual TLS with a qubit can be detectedvia various spectroscopic methods, most of which have relied on the tunability of the qubit frequency. We propose a novel method that requires only a microwave drive and dispersive readout, and thus also works fixed-frequency qubits. The proposed two-tone spectroscopy involves a microwave pulse of varying frequency and length to excite TLSs of unknown frequencies, followed by a second pulse at the qubit frequency. TLS parameters can be estimated from the qubit population as a function of the first pulse frequency and length.